Molecular Biology Reagents and Kits
Active Motif Nucleosome Preparation Kit
Active Motif's Nucleosome Preparation Kit is designed to prepare mono- and oligonucleosomes starting from cells. Nucleosomes comprise the smallest subunit of chromatin and consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Each nucleosome is separated by a linker of DNA. The Nucleosome Preparation Kit uses an enzymatic cocktail to digest the linker DNA. By adjusting the digestion time, it is possible to control the amount of mononucleosomes or oligonucleosomes extracted. Because isolated nucleosomes are more physiologically relevant than histones alone or synthetic peptides, they are ideally suited for use as substrates in the analysis of histone post-translational modifications, enzyme kinetics or inhibitor screening studies.
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Liver, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Boca Scientific Inc Buccalyse Rapid Buccal Cell DNA Isolation Kit, 50 Extractions, with 50 SK-1S Buccal Swabs
The BEK-50/SK1 kit includes 50 SK-1S Buccal Swabs and the Buccalyse DNA extraction kit for 50 samples. SK-1S Buccal Swabs are designed for high-yield DNA retrieval and convenient transport. Each SK-1S buccal swab is packaged with a 5ml plastic collection tube and cap. The Buccalyse DNA Extraction Kit, specifically designed for use with buccal swabs, includes one solution for a fast and simple one tube method for extracting DNA from buccal swabs. In only 20 minutes, this kit provides PCR-ready DNA that does not require centrifugation.
Cayman Chemical Fungal Lysergol 50mg
An ergot alkaloid and 5-HT1A, 5-HT2A, and 5-HT2C receptor agonist (EC50s = 73, 1.6, and 6.6 nM, respectively, in a FLIPR assay); induces contractions in isolated rat tail arteries (EC50 = 436.5 nM); enhances the antibacterial activity of nalidixic acid against susceptible and drug-resistant E. coli at 10 UG/ml
Biochain Institute Inc Control Genomic DNA - Cynomolgus Monkey Female, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Research Products International Corp Quick-DNA Plant/Seed Miniprep Kit, 50 Preps
The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology with Zymo-Spin IV-IR HRC filter. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
Ultra-high density BashingBeads are fracture resistant and chemically inert.
Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
Amsbio LLC MagSi-DNA mf
MagSi-DNA mf is ideal for developing your own application, such as: - Isolation of genomic, mitochondrial, or viral DNA from whole blood, cell lysates, human, animal, or plant tissue; isolation of RNA - Isolation of genomic, plasmid, or phage DNA from bacterial cultures and bacteria from clinical samples (blood, stool, swabs, etc.) - Clean-up of DNA from enzymatic reactions or chromatin immunoprecipitation (ChIP) | MagSi-DNA mf consists of magnetic silica beads with a highly dense core of magnetite. Due to their ferrimagnetic properties, the beads typically collect within 10 seconds in a magnetic field, but quickly loose magnetic remanence and are easily resuspended. The beads have minimal adhesion effects to plastics, making them perfectly suitable for use in microfluidic systems. The small particle size offers a very large active surface area for binding of nucleic acids, and the storage buffer has been optimized for maximum suspension time and ease of resuspension.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Brain, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biotium Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) (CALD1/1424R), CF647 conjugate, 0.1mg/mL
Caldesmon HMW is the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Heart, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Enzo Life Sciences AMPIPROBE Bacterial Vaginosis Extraction Controls (500µl)
The AMPIPROBE Candida Extraction Controls are designed to be used during the genomic DNA extraction of Atopobium vaginae, Gardnerella vaginalis, Lactobacillus spp., Megasphaera spp., and BVAB2. All performance specifications must be validated by end-user and will vary based on chosen extraction methods. Eluent from extraction can be used as extraction control for AMPIPROBE Bacterial Vaginosis Assay kit (ENZ-GEN205). Plasmids in Extraction Controls are present at higher concentrations compared to assay controls included in the qPCR kit. Extra care should be taken to avoid contamination. Applications: qPCR. Species reactivity: BVAB-2, Megasphaera-1, Lactobacillus spp., Gardnerella vaginalis, Atopobium vaginae.
Creative Bioarray CELL EX CELL MATRIX T75 FLASKS
Cell Extra cellular Matrix T75 flasks, 10 flasks per pack
MDI Membrane FAST ENDOFREE GIGAPREP 2RXN
Endotoxin free Gigaprep pDNA purification kits are certified for low endotoxin levels of < 0.1 EU/µg. These are specially designed to offer very high yields and purity with much reduced process time. The kit allows fast purification of large quantities of plasmid DNA, from culture pellet, in just 50 minutes. The high binding capacity Giga column with special membrane allows elution of highly concentrated plasmid DNA and does not require DNA precipitation and reconstitution, preventing yield losses and salt contamination.
Epoch Life Science Inc GenCatch™ Plasmid DNA Miniprep Spin Kit (50 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify plasmid DNA without phenol/chloroform extraction. It is based on binding of DNA to silica-based membranes in a chaotropic salt. High quality spin columns, collection tubes together with buffers and protocols. HIGHLIGHTS Fast: Up to 24 samples can be readily prepared in 30 minutes with minimum effort. Reliable: Used by labs around the world. References as far back as 2006 proves quality and customers' trust in our products and services. Flexible: Mini columns can be used in either centrifugation procedure or using a vacuum manifold. Convenient: No phenol extractions or ethanol precipitations required. Quality: DNA prepared by this kit is suitable for many downstream processes including PCR, ligation, restriction enzyme digestion, transformation, radioactive and fluorescent sequencingg, etc. 18 month shelf life when stored at RT. Typical Yield: 4-20ug of DNA from 2ml of overnight culture. Binding Capacity: 40ug.